Publications

Links to all papers from the lab are organized by year of publication.

Hyperstabilization of T cell microvilli contacts by chimeric antigen receptors. J Cell Biol. 2023 Feb 06; 222(3). Beppler C, Eichorst J, Marchuk K, Cai E, Castellanos CA, Sriram V, Roybal KT (co-corresponding author)Krummel MF. PMID: 36520493; PMCID: PMC9757849.
 
View in: PubMed 
 
Antigen-Dependent Inducible T-Cell Reporter System for PET Imaging of Breast Cancer and Glioblastoma. J Nucl Med. 2023 Jan; 64(1):137-144. Shin J, Parker MFL, Zhu I, Alanizi A, Rodriguez CI, Liu R, Watchmaker PB, Kalita M, Blecha J, Luu J, Wright B, Lapi SE, Flavell RROkada HTlsty TDRoybal KT (co-corresponding author), Wilson DM. PMID: 35981900.
 
View in: PubMed 
Synthetic cytokine circuits that drive T cells into immune-excluded tumors. Science. 2022 Dec 16; 378(6625):eaba1624. Allen GM, Frankel NW, Reddy NR, Bhargava HK, Yoshida MA, Stark SR, Purl M, Lee J, Yee JL, Yu W, Li AW, Garcia KC, El-Samad HRoybal KTSpitzer MHLim WA. PMID: 36520915.
 
View in: PubMed
 
Multidimensional control of therapeutic human cell function with synthetic gene circuits. Science. 2022 Dec 16; 378(6625):1227-1234. Li HS, Israni DV, Gagnon KA, Gan KA, Raymond MH, Sander JD, Roybal KT, Joung JK, Wong WW, Khalil AS. PMID: 36520914.
 
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Pooled screening of CAR T cells identifies diverse immune signaling domains for next-generation immunotherapies. Sci Transl Med. 2022 11 09; 14(670):eabm1463. Goodman DB, Azimi CS, Kearns K, Talbot A, Garakani K, Garcia J, Patel N, Hwang B, Lee D, Park E, Vykunta VS, Shy BRYe CJEyquem JMarson ABluestone JARoybal KT. PMID: 36350984.
 
View in: PubMed  
 
Diminished cell proliferation promotes natural killer cell adaptive-like phenotype by limiting FcεRIγ expression. J Exp Med. 2022 11 07; 219(11). Shemesh A, Su Y, Calabrese DR, Chen D, Arakawa-Hoyt J, Roybal KT, Heath JR, Greenland JRLanier LL. PMID: 36066491; PMCID: PMC9448639.
 
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Differential IL-12 signaling induces human natural killer cell activating receptor-mediated ligand-specific expansion. J Exp Med. 2022 Aug 01; 219(8). Shemesh A, Pickering H, Roybal KTLanier LL. PMID: 35758909; PMCID: PMC9240274.
 
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Modular design of synthetic receptors for programmed gene regulation in cell therapies. Cell. 2022 04 14; 185(8):1431-1443.e16. Zhu I, Liu R, Garcia JM, Hyrenius-Wittsten A, Piraner DI, Alavi J, Israni DV, Liu B, Khalil AS, Roybal KT. PMID: 35427499; PMCID: PMC9108009.
 
View in: PubMed  

 

SynNotch CAR circuits enhance solid tumor recognition and promote persistent antitumor activity in mouse models. Sci Transl Med. 2021 04 28; 13(591). Hyrenius-Wittsten A, Su Y, Park M, Garcia JM, Alavi J, Perry N, Montgomery G, Liu B, Roybal KT. PMID: 33910981; PMCID: PMC8594452.

 

View in PubMed | Press: Fierce Biotech, STAT, New Scientist, Nature Reviews Immunology

 

SynNotch-CAR T cells overcome challenges of specificity, heterogeneity, and persistence in treating glioblastoma. Sci Transl Med. 2021 04 28; 13(591). Choe JH, Watchmaker PB, Simic MS, Gilbert RD, Li AW, Krasnow NA, Downey KM, Yu W, Carrera DA, Celli A, Cho J, Briones JD, Duecker JM, Goretsky YE, Dannenfelser R, Cardarelli L, Troyanskaya O, Sidhu SS, Roybal KT (co-corresponding author)Okada HLim WA. PMID: 33910979; PMCID: PMC8362330

View in: PubMed | Press: STAT, New Scientist, Nature Reviews Immunology

DNA scaffolds enable efficient and tunable functionalization of biomaterials for immune cell modulation. Nat Nanotechnol. 2020 Dec 14. Huang X, Williams JZ, Chang R, Li Z, Burnett CE, Hernandez-Lopez R, Setiady I, Gai E, Patterson DM, Yu W, Roybal KT, Lim WA, Desai TA. PMID: 33318641.

View in: PubMed  
   

Precise T cell recognition programs designed by transcriptionally linking multiple receptors. Science. 2020 Nov 27; 370(6520):1099-1104. Williams JZ, Allen GM, Shah D, Sterin IS, Kim KH, Garcia VP, Shavey GE, Yu W, Puig-Saus C, Tsoi J, Ribas A, Roybal KT, Lim WA. PMID: 33243890.

View in: PubMed | Press: STAT, New Scientist

 

 

CRISPR-based screens uncover determinants of immunotherapy response in multiple myeloma. Blood Adv. 2020 Jul 14; 4(13):2899-2911. Ramkumar P, Abarientos AB, Tian R, Seyler M, Leong JT, Chen M, Choudhry P, Hechler T, Shah N, Wong SW, Martin TG, Wolf JL, Roybal KT, Pahl A, Taunton J, Wiita AP, Kampmann M. PMID: 32589729.

View in: PubMed 
 

convertibleCARs: A chimeric antigen receptor system for flexible control of activity and antigen targeting. Commun Biol. 2020 Jun 09; 3(1):296. Landgraf KE, Williams SR, Steiger D, Gebhart D, Lok S, Martin DW, Roybal KT, Kim KC. PMID: 32518350.

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Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies. Cell. 2020 04 30; 181(3):728-744.e21. Roth TL, Li PJ, Blaeschke F, Nies JF, Apathy R, Mowery C, Yu R, Nguyen MLT, Lee Y, Truong A, Hiatt J, Wu D, Nguyen DN, Goodman D, Bluestone JA, Ye CJ, Roybal K, Shifrut E, Marson A. PMID: 32302591.

 

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Paving New Roads for CARs. Trends Cancer. 2019 10; 5(10):583-592. Hyrenius-Wittsten A, Roybal KT. PMID: 31706506.

View in: PubMed   

 

NextGen cell-based immunotherapies in cancer and other immune disorders. Curr Opin Immunol. 2019 08; 59:79-87. Azimi CS, Tang Q, Roybal KT, Bluestone JA. PMID: 31071513.


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Clonal Deletion of Tumor-Specific T Cells by Interferon-y Confers Therapeutic Resistance to Combination Immune Checkpoint Blockade. Immunity. 2019 02 19; 50(2):477-492.e8. Pai CS, Huang JT, Lu X, Simons DM, Park C, Chang A, Tamaki W, Liu E, Roybal KT, Seagal J, Chen M, Hagihara K, Wei XX, DuPage M, Kwek SS, Oh DY, Daud A, Tsai KK, Wu C, Zhang L, Fasso M, Sachidanandam R, Jayaprakash A, Lin I, Casbon AJ, Kinsbury GA, Fong L. PMID: 30737146.

View in: PubMed   

 

Beyond the TCR, Antigen Discrimination in T Cells Continues in the LAT:GRB2:SOS Protein Condensate. Biophysical Journal. 2019 Feb 1; 116(3):530a. Shalini T. Low-Nam, Jenny JY Lin, Darren B. McAffee, Steven A. Alvarez, Scott D. Hansen, Kole T. Roybal, Jay T. Groves.

 

View in: Publisher Site

Identifying Factors in Multiple Myeloma Controlling Response to B-Cell Maturation Antigen (BCMA)-Targeted Immunotherapy Using CRISPR-Based Functional Genomics. Blood. 2018 Nov 29; 132(Supplement 1):1926-1926. Poornima Ramkumar, Jaime Leong, Meghan Seyler, Stratton J Georgoulis, Axel Hyrenius Wittsten, Priya Choudhry, Kole Roybal, Arun P Wiita, Martin Kampmann.

 

View in: Publisher’s website

 

Refining cell therapy. Science. 2018 Mar 09; 359(6380):1112-1113. Roybal KT. PMID: 29590036.

View in: PubMed   

Synthetic Immunology: Hacking Immune Cells to Expand Their Therapeutic Capabilities. Annu Rev Immunol. 2017 04 26; 35:229-253. PMID: 28446063.

View in: PubMed   

CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells. Sci Rep. 2017 04 07; 7(1):737. PMID: 28389661.

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Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Cell. 2016 Oct 06; 167(2):419-432.e16. Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, Walker WJ, McNally KA, Lim WA. PMID: 27693353; PMCID: PMC5072533.

 

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Computational spatiotemporal analysis identifies WAVE2 and cofilin as joint regulators of costimulation-mediated T cell actin dynamics. Sci Signal. 2016 Apr 19; 9(424):rs3. Roybal KT, Buck TE, Ruan X, Cho BH, Clark DJ, Ambler R, Tunbridge HM, Zhang J, Verkade P, Wülfing C, Murphy RF. PMID: 27095595; PMCID: PMC4871116..

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Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Cell. 2016 Feb 11; 164(4):780-91. Morsut LRoybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim WA. PMID: 26830878; PMCID: PMC4752866.


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Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits. Cell. 2016 Feb 11; 164(4):770-9. Roybal KT, Rupp LJ, Morsut L, Walker WJ, McNally KA, Park JS, Lim WA. PMID: 26830879; PMCID: PMC4752902.


View in: PubMed   

 

Cellular Structures Controlling T Cell Signaling in Time and Space. Encyclopedia of Cell Biology. 2016 Jan 1; 677-686. Wülfing WC, Roybal KT.


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Remote control of therapeutic T cells through a small molecule-gated chimeric receptor. Science. 2015 Oct 16; 350(6258):aab4077. Wu CY, Roybal KT, Puchner EM, Onuffer J, Lim WA. PMID: 26405231; PMCID: PMC4721629.


View in: PubMed   

 

Modest Interference with Actin Dynamics in Primary T Cell Activation by Antigen Presenting Cells Preferentially Affects Lamellal Signaling. PLoS One. 2015; 10(8):e0133231. Roybal KT, Mace EM, Clark DJ, Leard AD, Herman A, Verkade P, Orange JS, Wülfing C. PMID: 26237588; PMCID: PMC4523178.


View in: PubMed   

 

Early Signaling in Primary T Cells Activated by Antigen Presenting Cells Is Associated with a Deep and Transient Lamellal Actin Network. PLoS One. 2015; 10(8):e0133299. Roybal KT, Mace EM, Mantell JM, Verkade P, Orange JS, Wülfing C. PMID: 26237050; PMCID: PMC4523204.


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Synthetic biology approaches to engineer T cells. Curr Opin Immunol. 2015 Aug; 35:123-30. Wu CY, Rupp LJ, Roybal KTLim WA. PMID: 26218616; PMCID: PMC4553068.


View in: PubMed   

 

Cellular structures controlling T cell signaling in time and space. Encyclopedia of Cell Biology. 2015; 677-686. Wülfing C, Roybal KT.

 

View in: Publishers website

 

IFN-α suppresses GATA3 transcription from a distal exon and promotes H3K27 trimethylation of the CNS-1 enhancer in human Th2 cells. J Immunol. 2014 Jun 15; 192(12):5687-94. Huber JP, Gonzales-van Horn SR, Roybal KT, Gill MA, Farrar JD. PMID: 24813204; PMCID: PMC4104489.


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Enhancement of anti-tumor CD8 immunity by IgG1-mediated targeting of Fc receptors. MAbs. 2014 Jan-Feb; 6(1):108-18. Furlan SN, Mandraju R, Brewer T, Roybal K, Troutman TD, Hu W, Palm NW, Unni A, Pasare C. PMID: 24284965;PMCID: PMC3929435.

 

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The actin-driven spatiotemporal organization of T-cell signaling at the system scale. Immunol Rev. 2013 Nov; 256(1):133-47. Roybal KT, Sinai P, Verkade P, Murphy RF, Wülfing C. PMID: 24117818.


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Differential regulation of the period genes in striatal regions following cocaine exposure. PLoS One. 2013; 8(6):e66438. Falcon E, Ozburn A, Mukherjee S, Roybal K, McClung CA. PMID: 23776671; PMCID: PMC3679086.


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New TACTICS for finding Numb. Immunol Cell Biol. 2013 Jan; 91(1):1-2. Roybal KT, Wülfing C. PMID: 23183270.


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Mycobacterial shuttle vectors designed for high-level protein expression in infected macrophages. Appl Environ Microbiol. 2012 Oct; 78(19):6829-37. Eitson JL, Medeiros JJ, Hoover AR, Srivastava S, Roybal KT, Aínsa JA, Hansen EJ, Gumbo T, van Oers NS. PMID: 22820329; PMCID: PMC3457510.

 

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The CD3 zeta subunit contains a phosphoinositide-binding motif that is required for the stable accumulation of TCR-CD3 complex at the immunological synapse. J Immunol. 2011 Jun 15; 186(12):6839-47. DeFord-Watts LM, Dougall DS, Belkaya S, Johnson BA, Eitson JL, Roybal KT, Barylko B, Albanesi JP, Wülfing C, van Oers NS. PMID: 21543646; PMCID: PMC3110614.


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Tentative and transient natural killer cell polarization balances the requirements for discriminatory recognition and cytolytic efficacy. Commun Integr Biol. 2010 Nov; 3(6):545-8. Sinai P, Roybal KT, Wülfing C. PMID: 21331235.

View in: PubMed

 

Inhibiting the inhibitor of the inhibitor: blocking PKC-theta to enhance regulatory T cell function. Sci Signal. 2010 Jul 27; 3(132):pe24. Roybal KT, Wülfing C. PMID: 20664063.

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Spatiotemporal patterning during T cell activation is highly diverse. Sci Signal. 2009 Apr 07; 2(65):ra15. Singleton KL, Roybal KT, Sun Y, Fu G, Gascoigne NR, van Oers NS, Wülfing C. PMID: 19351954.

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Spatiotemporal patterning of T cell signaling as an important regulator of T cell activation at the systems level. The FASEB Journal. 2008 Mar 1; 22:1064.16-1064.16. Christoph Wuelfing, Kentner L. Singleton, Kole T. Roybal, Yi Sun. .

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Mania-like behavior induced by disruption of CLOCK. Proc Natl Acad Sci USA. 2007 Apr 10; 104(15):6406-11. Roybal K, Theobold D, Graham A, DiNieri JA, Russo SJ, Krishnan V, Chakravarty S, Peevey J, Oehrlein N, Birnbaum S, Vitaterna MH, Orsulak P, Takahashi JS, Nestler EJ, Carlezon WA, McClung CA. PMID: 17379666.

 

View in: PubMed   

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News

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'Smart' immune cells kill tumours and stop them regrowing in mice
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Attacking glioblastoma and other solid tumors with CAR-Ts that target multiple antigens
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Tweaking Mother Nature, biologists aim for better cancer-fighting cells
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'Cell Bots' Chase Down Cancer, Deliver Drugs Directly to Tumors
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Synthetic Notch receptors were featured in Notable Advances 2016
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Kole Roybal receives the inaugural Sartorius & Science Magazine Prize in Regenerative Medicine and Cell Therapy 2018
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Boosting the immune system to fight cancer
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Engineering T cells with Customized Therapeutic Response Programs with Synthetic Notch Receptors
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